Elicitation of antioxidant metabolites in Musa species in vitro shoot culture using sucrose, temperature and jasmonic acid

Abstract In vitro culture is not only known for the mass propagation of medicinal plants, it can also be used to boost accumulation useful metabolites. This work was aimed at studying potential elicitation on antioxidant secondary metabolites in different Musa species accessions. Shoot cultures selected six spp. accessions were micropropagated Murashige and Skoog mineral-based medium, supplemented with indole-3-acetic acid 6-benzylaminopurine. Different temperatures (15, 20 26 °C), doses sucrose (30–50 g/L) jasmonic concentrations (JA, 50–200 µM) as elicitors medium. High-performance thin-layer chromatography (HPTLC) coupled effect-directed analysis (EDA) compare characterize all 71 samples from reveal compound zones antioxidant, antidiabetic anticholinesterase activities. All plant tissue experiments increased total phenolic content (TPC) activity Extracts plants grown °C gave highest TPC activity. Addition JA (200 higher contents (40 g/L or 45 well. Not compounds but activities detected elicited The system presented this study could serve a sustainable, consistent reliable alternative means bioactive supply production biopharmaceuticals.